摘要
蛋白质组学霰弹法策略是大规模分析复杂蛋白质混合物的有力方法,它以多维色谱和串联质谱为核心技术,随着数据库和计算机分析数据的能力而发展。本文综述了它的实验流程、MS/MS(tandemmassspectrometry,MS/MS)数据解释的方法、过程和解释中存在的问题以及在实际研究中的应用。
Shotgun strategy for proteomics were as a powerful approach for high-throughput analysis of complex protein mixtures. Based on the integration of multi-dimensional chromotograph and mass spectrometry and evolving with the proteomic informatics, its experimental protocol, interpretation of MS/ MS data and application were reviewed and discussed.
出处
《蚕桑通报》
2006年第3期6-11,共6页
Bulletin of Sericulture
关键词
霰弹法
蛋白质组学
质谱分析
应用
Shotgun strategy
Proteomics
Mass spectrometry
Application
