大鼠致痫后血清和脑组织皮质醇水平的测定及其意义

Cortisol Levels in the Serum and the Brain Regions in Convulsive Rats

应用放免技术测定了马桑内酯致痫和对照组大鼠大脑皮层和海马脑组织皮质醇含量及血清皮质醇水平结果显示，血清对照组为２．７７±２．０７ｎｇ／ｍｌ，致痫组为０．９８±０．７５ｎｇ／ｍｌ，两组比较无显著差异（Ｐ＞０．０５）；海马正常对照组为２５．７７±１２．９８ｎｇ／ｇ湿重，致痫组为０．９８±０．４２ｎｇ／ｇ湿重组织，海马致痫组显著降低（Ｐ＜０．０１）；大脑皮层对照组为８．８３±１．９６ｎｇ／ｇ湿重，致痫组为０．３３±０．１１ｎｇ／ｇ湿重组织，大脑皮层致痫组降低极为显著（（Ｐ＜０．００１）。表明致痫后大鼠大脑皮层、海马脑组织皮质醇含量均明显降低，提示皮质醇在癫痫的发病中起重要作用。

Studying the changes of the cortisol levels in serum and the brain tissue of convulsive rats injected coriaria lactone intral-lateral ventricularly(ilv),the control rats received saline.The important finding was that after convulsion there was,as compared with control,a marked attenuation of the cortisol levels in the cerebral cortex(CC)and the hippocampus(hip).(control:CC: 8.83±1.96ng/g wet tissue,hip:25.77±12.98ng/g wet tissue,serum:2.77±2.07ng/ml.convulsive:CC:0.33±0.1ng/g wet tissue,P<0.001.hip:0.97±0.42ng/g wet tissuemP<0.001,serum:0.89±0.75ng/ml,P>0.05).