红景天多糖对骨髓抑制贫血小鼠外周血及骨髓细胞周期的影响

Effect of Rhodiola Polysaccharide on Peripheral Blood Cells and Bone Marrow Cell Cycle of myelosuppressed Anemia Mice

目的观察红景天多糖对骨髓抑制贫血小鼠外周血及骨髓细胞周期的影响,并探讨其造血调控作用机制。方法用全自动血细胞分析仪、白细胞计数法、流式细胞术(FCM)分别检测红景天多糖对骨髓抑制贫血小鼠外周血、骨髓有核细胞、骨髓细胞周期的影响。结果中剂量和高剂量红景天多糖能明显升高外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)及骨髓有核细胞(BMCs)数,但对血小板的作用不明显。高剂量组可促进骨髓G0/G1期细胞向S期细胞以及S期细胞向G2/M期细胞的转化,增殖指数(PI)也明显升高。结论红景天多糖可能通过促使骨髓抑制贫血小鼠骨髓细胞通过G1期的限制点,进入细胞增殖周期,加速骨髓G0/G1期细胞向S期细胞、S期细胞向G2/M期细胞的转化,促进骨髓造血细胞增殖,提高外周血象,促进骨髓造血功能的恢复。

Objective To examine the effect of rhodiola polysaccharide on peripheral blood cells and cell cycle of BMCs in myelosuppressed anemia mice, and to explore its mechanism in hematopoieric regulation. Methods The effect of rhodiola polysaccharide on peripheral blood cells, BMCs, and cell cycle of BMCs in myelosuppressed anemia mice was detected by automatic blood cell analysator, white blood count and flow cytometry （FCM） respectively. Results It was found that medium- and high-dose rhodiola polysaccharide obviously elevated white blood cells,red blood cells,hemoglobin and BMCs, but had not effect on blood platelet. High-dose rhodiola polysaccharide significantly promoted the transition of BMCs from G0/G1 to S phase and S to G2/M phase, and that both medium-and high-dose rhodiola polysaccharide obviously elevated the proliferation index. Conclusion These data suggest that rhodiola polysaccharide may promote the recovery of hematopoietic function of the myelosuppressed anemia in mice by ending off G0/G1-phase arrest, accelerating G0/G1-S phase and S- G2/M phase transition, promoting the proliferation of bone marrow hemopoietic cells.