摘要
选用引物JYF5/JYR5对湖北疑似柑橘溃疡病标本进行了PCR鉴定和确证,同时改进了DNA抽提方法,优化了PCR条件,并与南京柑橘溃疡病菌株进行分子克隆比较。结果表明,南京菌株NJ-XC分离物扩增片段与Silva等报道的相应序列相似性为99%(411/413);湖北标本菌株HB-X0扩增片段与报道的相应序列相似性为100%(413/413),二者存在一定的差异。此外发现引物JYF5/JYR5能扩增叶表的根癌土壤杆菌,其片段大小为575bp。
In this paper, a citrus sample from Hubei was identified by PCR Assay. At the same time, the method of DNA extraction was developed, and it was cloned and sequenced to make a comparison with Xac from Nanjing. The result showed that the two different strains had certain discrepancy in sequences. The comparability between the Nanjing sequence and that reported by Silva （2002）was 99 percent （411/413）;the comparability between the Hubei sequence and the reported was 100 percent（413/413）. Under the same primer, the amplified fragments of and unknown stain derived from the disease spots isolation showed high comparability of 93 percent（484/515 ）with one section of Agrobacterium tumefociens sequences（575bp）.
出处
《湖北农业科学》
北大核心
2006年第5期542-545,共4页
Hubei Agricultural Sciences
基金
湖北省出入境检验检疫局重点科技项目(HUBE2005-04)
