细胞内La表达对乙肝病毒复制的影响研究

The effect of La protein on replications of HBV in cultured cell

目的:研究细胞内La的表达对HBV病毒复制的影响。方法:针对人La蛋白序列设计并合成特异性的SiR-NA,转染稳定表达HBV的HepG2.2.15细胞,实时荧光定量PCR测定HepG2.2.15细胞中La mRNA变化水平及HBV DNA复制水平。结果:特异性SiRNA干扰La蛋白的mRNA表达,使La在HepG2.2.15细胞中的表达降低;HepG2.2.15细胞分泌在培养上清液中的HBV DNA水平显著下降,相关性分析结果显示,HBV DNA变化水平与La mRNA变化水平呈正相关。结论:细胞内La可以保护HBV RNA免受核酸酶的破坏、促进HBV病毒复制。

Objective：To prove whether La protein could effect the replications of the HBV in cultured cells. Methods：Three specific SiRNAs for human La protein was obtained by transcription in vitro. After transfection with the SiRNAs into HepG2. 2. 15 cell, the levels of La protein mRNA and HBV DNA were detected by real-time PCR. Results：The HBV DNA secreted by the cell transfected with the SiRNAs was reduced with reduction of the mRNA of the La protein. Conclusion ： The La protein can protect the replications of the HBV in cultured cells.