不同浓度泌乳素对葡萄膜炎患者外周血淋巴细胞增殖活化的影响

The effects of different concentrations of prolactin on the proliferation and activation of peripheral blood lymphocytes in the uveitic patients

目的：探讨不同浓度泌乳素（Prolactin，PRL）对葡萄膜炎患者外周血单个核细胞增殖及活化的影响，研究PRL在葡萄膜炎自身免疫中的作用。方法：用化学发光法测定葡萄膜炎患者及正常对照者血清PRL的水平；分离获得葡萄膜炎患者及正常对照者外周血单个核细胞，与S抗原及不同浓度PRL进行培养，108小时后用双标记免疫荧光染色流式细胞检测技术测定CD3^＋CD25^＋细胞的百分数；培养96小时后加入^3H-胸腺嘧啶核苷，12小时后测定每分钟脉冲数；观察在S抗原的刺激下，不同浓度的PRL对葡萄膜炎患者外周血单个核细胞增殖活化的影响。结果：葡萄膜炎患者血清PRL水平与正常人差异无显著意义（t=1．963，P=0．051）。葡萄膜炎患者对S抗原呈阳性反应时，12．5-1000μg/L的PRL有促进葡萄膜炎患者外周血单个核细胞增殖与活化的趋势，在200μg／L的浓度时促进作用最显著（活化t=2．736，P=0．012；增殖t=2．547，P=0．036），1000μg/L时趋势下降。葡萄膜炎患者对S抗原呈阴性反应时，则没有上述作用。结论：在小样本研究中葡萄膜炎患者血清中PRL水平与正常对照者相比差异无显著意义；中等浓度的PRL（200μg／L）能同S-Ag发挥共刺激作用，促进葡萄膜炎患者单个核细胞的增殖与活化，过高浓度（1000μg／L）则失去促进作用。

Objective：To research the autoimmune mechanism of prolactin（PRL） in uveitis by observing prolaictin＇s effects on peripheral blood mononuelear cells （PBMC） proliferation and activation at different concentration. Methods：1. The serum prolaetin levels of uveitis and control were checked by chemical spectrometer. 2. To research the effects of different concentration PRL on uveitis PBMC＇s proliferation and activation under the soluble antigen（S-Ag） stimulation. PBMC separated from uveitis and control,were cultured with S-Ag and different concentration PRL,and then were assayed the percentage of CD3^＋CD25^＋ positive cell through doublestain immunofluorescence flow cytometry after 108 h. After cultured 96 h, ^3H-TdR was mixed and counts per minute were assayed after 12 h. Results： 1. There was no significance between the uveitis and control refer to the serum PRL level（ t = 1. 936,P = 0. 051 ）. 2. When uveitis＇ PBMC was positive to S-Ag, there had promote proliferation and activation of PBMC with PRL concentration at 12.5- 1 000μg/L,with the media concentration at 200μg/L,while it became weakened at 1 000μg/L（activation t =2.736,P =0. 012;proliferation t =2. 547 ,P =0. 036）. When uveitis＇ PBMC was negative to S-Ag,there had no action as before. Conclusion： 1. There is no significance between the uveitis and control refer to the serum PRL level. 2. Media concentration PRL（ 200μg/L） and S-Ag have costimtdation to promote PBMC proliferation and activation in uveitis ,though high concentration PRL loses the function.