阻塞性睡眠呼吸暂停犬模型肿瘤坏死因子-α与左心室功能的关系

Relationship Between TNF-alpha and Left Ventricular Function in Dogs with Obstructive Sleep Apnea

目的:研究阻塞性睡眠呼吸暂停综合征(OSAS)犬模型血清中肿瘤坏死因子-α(TNF-α)含量的变化及其与心功能的关系。方法:设计OSAS犬模型,实验在6只正常杂种犬上进行。用ELISA法检测其血清中TNF-α含量。心脏彩超测量左室射血分数(LVEF)、左室舒张末期容积(LVEDV)。结果:TNF-α水平较实验前升高(P<0.05),LVEF、LVEDV、SV、HR、E/A值均较干预前降低(P<0.05)。TNFα-与LVEF呈负相关(r=-0.883,P<0.05),与LVEDV呈正相关(r=0.735,P<0.05)。结论:TNFα-可能在OSAS的心血管的发病中起着重要作用。

Objective： To study the effects of tumor necrosis factor-alpha（TNF-α） on the cardiovascular function in dogs with obstructive sleep apnea syndromes （OSAS）. Methods： Experiments were performed in six mature male Mongrel OSAS dogs. Serum concentrations of TNF-α of each dog were measured by ELISA immunoassay. The cardiovascular function indices as left ventricle ejection fraction （LVEF）, left ventricle end-diastolic volume （LVEDV）, stroke volume （SV）, heart rate （HR） and E/A were measured by using echocardiography. Results： Serum concentrations of TNF-α increased significantly （P〈0. 05）, and LVEF, LVEDV, SV, HR and E/A decreased compared with that of three months ago respectively （all P〈0.05）. TNF-α was negatively related withLVEF （r=-0. 883,P〈0. 05） but positively with LVDEV （r=0. 735,P〈0. 05）. Conclusion： The increase of serum concentrations of TNF-α may play an important role in the pathogenesis of cardiovascular diseases in OSAS.