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一种有效的免疫学检测技术——ELISPOT 被引量：3

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摘要 20世纪80年代中期,国外的科研工作者根据ELISA技术的基本原理,建立了体外检测特异性抗体分泌细胞和CK分泌细胞的固相酶联免疫斑点技术(ELISPOT)。因其具有较高的特异性、稳定性和敏感性,已广泛应用于免疫学各领域。文章详细介绍了ELISPOT技术的发展和应用,并分析了其优缺点、操作注意事项及可行性。
作者 熊仲良 邓小红 曾政
机构地区 重庆市动物卫生监督总站 重庆工商大学药物化学与化学生物学研究中心
出处 《动物医学进展》 CSCD 2006年第9期101-104,共4页 Progress In Veterinary Medicine
关键词 酶联免疫斑点技术 免疫学 发展和应用
分类号 S854.43 [农业科学—临床兽医学]
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参考文献21

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  • 2Czerkinsky C,Andersson G,Ekre H P,et al,Reverse ELISPOT assay for clonal analysis of cytokine production.I.Enumeration of gamma-interferon-secreting cells[J].Immunol Methods,1988,110:29-32.
  • 3Kreher C R,Dittrich M T,Guerkov R,et al.CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays[J].Journal of Immunological Methods,2003,278:79-93.
  • 4Mccutcheon M,Wehner N,Wensky A,et al.A sensitive ELISPOT assay to detect low-frequency human T-lymphocytes[J].Immunol Methods,1997,210:149-166.
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  • 6Samri A,Durier C,Urrutia A,et al.Evaluation of the interlaboratory concordance in quantification of human immunodeficiency virus-specific T cells with a gamma interferon enzyme-linked immunospot assay[J].Clin Vaccine Immunol,2006,13(6):684-97.
  • 7Nordstrom I,Ferrua B.Reverse ELISPOT assay for clonal analysis of cytokine production[J].Immunol Methods,1992,150:199-206.
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  • 9Vaquerano J E,Peng M,Chang J W,et al.Digital quantification of the enzyme-linked immunospot(ELISPOT)[J].Biotechniques,1998,25(5):4830-4836.
  • 10Okamoto Y,Abe T,Nixa T,et al.Development of a dual color enzyme-linked immunospot assay for simutaneous detection of murine T helper type 1-and T helper type 2-cells[J].Immunopharmacology,1998,39:106-116.

同被引文献34

  • 1鲁俊鹏,罗满林,邹潍力,李立霞,李瑞亭.牛结核病两种检测方法的比较[J].中国动物检疫,2006,23(10):30-31. 被引量：4
  • 2孟成艳,金嘉琳,张文宏.酶联免疫斑点法在结核性脑膜炎诊断中的应用[J].中华传染病杂志,2006,24(4):276-277. 被引量：12
  • 3乐军,梁莉,李苏辉,张跃建,肖和平.酶联免疫斑点试验快速诊断结核分枝杆菌感染的临床应用价值[J].中华检验医学杂志,2006,29(11):1005-1008. 被引量：70
  • 4Majid A M, Ezelle H, Shah Set al. Evaluating replication-defective ve- sicular stomatitis virus as a vaccine vehicle [ J ]. J Viro|, 2006;80 (14) :6993-7008.
  • 5Samri A, Durier C, Urrutia A et al. Evaluation of the interlaboratory concordance in quantification of human immunodeficiency virus-specif- ic T cells with agamma interferon enzyme-linked immunospot assay [ J ]. Clin Vaccine Immunol,2006 ; 13 (6) :684-697.
  • 6Dubey S, Clair J, Fu T M et al. Detection of HIV vaccine-induced cell- mediated immunity in HIV-seronegative clinical trial participants using an optimizeld and validated enzyme-linked immunospot assay [ J ]. Ac- quir Immune Defic Syndr,2007 ;45 ( 1 ) :20-27.
  • 7Zoe Moodie , Yunda Huang, Lin Gu et al. Statistical positivity criteria for the analysis of ELISPOT assay data in HIV-1 vaccine trials[J]. J Immunol Methods ,2006 ;315 (1-2) : 121-132.
  • 8Sedgwick JD, Holt PG, A solid-phase immunoenzymatic technique for the enumeration of specific antibodysecreting cells[J]. J Immunol Methods, 1983, 57 (1-3): 301-309.
  • 9Czerkinsky CC, Nilsson LA, Nygren H, et al, A solid-phase enzyme-linked immunospot(ELISPOT) assay for enumeration of specific antibody-secreting cells[J].J Immunol Methods, 1983,65(1-2):109-121,
  • 10Czerkinsky CC, Andersson G, Ekre HP, et al. Reverse ELIS- POT assay for clonal analysis of cytekine production I. Enumeration of gamma-interferon-secreting cells[J].Immunol Methods, 1988, 110(1): 29-36.

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动物医学进展
2006年 第9期

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