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T-bet/GATA-3的比值在哮喘大鼠免疫失衡中的意义 被引量：4

Significance of T-bet/GATA-3 ratio in immune disbalance of asthmatic rats
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摘要 目的:探讨转录因子T-bet/GATA-3比值在支气管哮喘免疫失衡中的意义.方法:SPF级SD大鼠随机分为对照组和哮喘组,每组12只.用逆转录-聚合酶链反应(RT-PCR)测定法和Western blot法分别检测T-bet,GATA-3 mRNA和蛋白在肺组织的表达.结果:大鼠肺组织中对照组和哮喘组T-bet mRNA表达分别为0.71±0.19,0.08±0.12;T-bet蛋白表达分别为0.72±0.22,0.18±0.06,两组比较均有显著性差异(P<0.01).大鼠肺组织中对照组和哮喘组GATA-3 mR-NA表达分别为1.06±0.25,1.56±0.28;GATA-3蛋白分别为1.04±0.44,2.25±0.74,两组比较均有显著性差异(P<0.01);对照组T-bet/GATA-3 mRNA表达量的比值0.73±0.32,明显高于哮喘组0.06±0.09(P<0.01),对照组T-bet/GATA-3蛋白表达量的比值0.75±0.25,高于哮喘组0.09±0.04(P<0.01).结论:转录因子T-bet/GATA-3 mRNA和蛋白表达量比值在哮喘中明显降低,可作为评价哮喘免疫失衡的客观指标之一. AIM： To investigate the significance of the T-bet/ GATA-3 ratio in the immune disbalance of the asthmatic rats. METHODS： Twenty-four male SD rats were randomly divided into a control group and an asthma group （ n = 12 per group）. The expressions of T-bet and GATA-3 mRNA and protein in the lungs were detected by revere transcriptase-polymerase chain reaction （RT-PCR） and Western blot respectively. RESULTS： In the control group, the expression of T-bet mRNA was 0.71± 0.19, which was significantly elevated compared with that of the asthma group（0.08±0. 12, P 〈0.01）; the levels of T-bet protein expression were 0.72 ±0.22 in the control group and 0.18±0.06 in the asthma group （ P 〈 0.01 ） ; The levels of GATA-3 mRNA expression were 1.06±0.25 in the control group and 1.56± 0.28 in the asthma group （P 〈 0.01 ） ; in the asthma group, GATA-3 protein expression （ 1.04± 0.44） increased compared with that of the control group（2.25± 0.74, P 〈0.01 ） ; in the control group, the ratio of gene expression of T-bet/GATA-3 （ 0.73± 0.32 ） was significantly increased compared with that of the asthma group （0.06±0.09, P 〈0.01 ） , and there was a significant difference between the control group （ 0.75 ± 0.25 ） and the asthma group （0.09 ±0.04） in the ratio of protein expression of T-bet/GATA- 3 （P 〈 0.01 ）. CONCLUSION： The mRNA and protein ratios of T-bet/GATA-3 decrease significantly in asthma, which may be one of the objective indexes to evaluate the immune disbalance in asthma.
作者 卞涛 殷凯生 金淑贤 周锦勇 马秀琴 德伟
机构地区 南京医科大学第一附属医院呼吸科 南京医科大学生化实验室
出处 《第四军医大学学报》 北大核心 2006年第18期1690-1693,共4页 Journal of the Fourth Military Medical University
关键词 哮喘 转录因子 T淋巴细胞 asthma transcription factors T-lymphocytes
分类号 R562 [医药卫生—呼吸系统]
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参考文献10

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共引文献203

同被引文献55

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引证文献4

二级引证文献33

第四军医大学学报
2006年 第18期

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