微小颗粒骨复合细胞移植治疗大鼠骨缺损

Treatment of rat cranial defect with autogenetic minimal morselized bone and allogenic cells

目的:观察自体微小颗粒骨复合骨髓基质细胞(MSC)或成骨细胞移植修复大鼠骨缺损的治疗效果.方法:制作大鼠颅骨缺损动物模型,培养同种异体新生大鼠的MSC及成骨细胞,复合自体颗粒骨后植入骨缺损区,X线摄片观察骨愈合情况;RT-PCR检测转化生长因子β1(TGF-β1),碱性成纤维细胞生长因子(bFGF)表达水平.结果:植入微小颗粒骨复合成骨细胞组颅骨缺损愈合最快(7.33±0.44)wk,TGF-β1,bFGF表达出现早,与其他两组比较差异显著(P<0.05).植入微小颗粒骨复合MSC组颅骨缺损愈合时间居中(8.67±0.69)wk,TGF-β1,bFGF表达早于单纯微小颗粒骨组(P<0.05).单纯微小颗粒骨植入组颅骨缺损愈合慢(11.67±1.52)wk,TGF-β1,bFGF表达出现晚.结论:微小颗粒骨复合细胞移植修复颅骨缺损,细胞因子表达早,缺损愈合明显加快.

AIM： To investigate the combined treatment effects of autogenetic minimal morselized bone and allogenic cells on rat cranial defect. METHODS： Cranial defect in rat models was repaired with autogenetic minimal morselized bone graft and allogenic cell transplantation [ osteoblasts or marrow stormal cells （MSC） ]. X ray was taken to understand the union condition, and RT-PCR was performed to examine the mRNA expressions of TGF-β1 and bFGF. RESULTS： In the order of combined treatment group A （morselized bone and osteoblast graft）, combined treatment group B （morselized bone and MSC graft ） , combined treatment group C（ minimal morselized bone only） , the union time of cranial defect prolonged as （7.33±0.44） wk, （ 8.67 ± 0.69 ） wk and （11.67 ± 1.52） wk, and the expression of TGF-β1 and bFGF emerged in the order （ P 〈 0.05 between every 2 groups ）. CONCLUSION： The combined therapy of minimal morselized bone and allogenic cells accelerate the repairment of cranial defect and the expression of cell factors.