巴通体的生物学观察及巴通体感染与肠组织病变关系的初步研究

Structural Study of Bartonella and Intestinal Pathology of Bartonella Infection

目的观察巴通体的组织形态学和超微结构,并将分离的巴通体感染实验动物,观察巴通体在血细胞内的发育特征,探讨巴通体感染和肠组织病理变化之间的相互关系,为临床诊治提供依据。方法取患者肠组织进行病理切片,HE染色后光镜观察。将患者的外周血及感染大鼠的腹腔液经固定后进行超薄切片,透射电镜观察。将患者排泄物中分离的感染有病原体的血细胞感染Wistar大鼠,观察血细胞感染率与肠组织病理变化之间的关系。结果实验大鼠的肠组织病理变化特征与患者的相似。粒细胞、单核/巨噬细胞胞浆呈空泡化,内有包涵体和巴通体;红细胞血色素下降,胞内呈空泡状,在大的空泡内见到不同发育期的巴通体;肠组织充血、变性和坏死。结论成功地建立了巴通体实验动物模型;宿主感染巴通体后,主要累及血细胞和肠组织,且血细胞内巴通体的感染率与肠组织病变的严重程度呈正相关。

Objective To observe the morphological and ultrastructural structure of Barton, ella and explore the relationship between the Barton, ella infection and intestinal tissue pathology based on experimental animal model. Methods The intestinal tissues from Bartonella infected patient and rat were used to prepare samples for light microscopic and electron microscopic analysis. Results The infected patients and rats had similar pathologic characteristics in intestinal tissue. There were vacuoles in granulocytes, monocytes, and macrophages,containing inclusion bodies and BartoneUa pathogens. The hemoglobin level was decreased in the red cells. Vacuoles were also observed in the red cells containing Bartonella at different developmental stages. There was positive correlation between the infective rate of Bartonella and the severity of intestinal pathology. Conclusion Both human and rat can be infected by Bartonella. The pathogen mainly invades graulocytes, monocytes, red blood cells and intestinal tissue. The pathology of the injfected intestinal tissue is correleated with the infective rate of Bartonella.