UU/CT在不同人群女性下生殖道中的检测分析

To Study UU/CT in Lower Genital Tract Among Different Female

目的了解在解脲脲原体(UU)和沙眼衣原体(CT)女性下生殖道感染的患者中的感染状况,为临床正确诊断和治疗提供依据。方法对601例拟诊为下生殖道感染的女性患者和306例正常体检的女性进行临床检查,阴道分泌物的pH值检测,假丝酵母菌、滴虫检测,胺试验和线索细胞检查,同时采用荧光定量PCR测定解脲脲原体、淋球菌、沙眼衣原体。结果在601例女性下生殖道感染的患者中,解脲脲原体阳性421例(70.1%),沙眼衣原体感染153例(25.5%),均较对照组(分别为41.2%和7.2%)明显升高,P均<0.05。此外,UU以单独感染的状态为主,也可与其它病原体呈混合感染状态;而CT以混合感染为主。结论解脲脲原体、沙眼衣原体可能是当前女性下生殖道感染的主要致病微生物,有必要进行常规的检查,以便正确使用有效的药物治疗。

Objective To investigate the Ureaplasma urealyticum （UU） and Chlamydia trachomatis （CT） infection among the Guangzhou females with lower genital tract infection and provide correct clinical diagnosis and treatment. Methods Physical examination was undertaken for all patients with vulvae itching and vaginal discharge. Swabs of vaginal discharge were tested for pH, amine, candida, trichomonas and clue cells. Samples were collected from cervix with scaled cotton for the detection of N. gonorrhoea, Ureaplasma urealyticum and Chlamydia trachomatis by fluorescent quantitative PCR （FQ-PCR）. The weight of secretion was determined by differential subtraction method. Results The detection rate of U. urealyticum and Chlamydia trachomatis were 70.1% and 25.5%, respectively, in 601 women who were suffering from lower genital tract infection. On the other hand, the detection rates were 41.2% and 7.2% in 306 healthy women. The differences were significant （P〈0.05）. U. urealyticum infection was single or mixed, while Chlamydia trachomatis infection were usually mixed. Conclusion The main pathogenic microorganism in women who suffering from lower genital tract infection were U. urealyticum and Chlamydia trachomatis. It is necessary to carry out conventional examination for the clinical diagnosis and treatment.