摘要
常用的转基因检测方法可分为两个方向,一是以检测外源基因为目标,如多聚酶链式反应分析法(PCR),二是以检测外源蛋白为目标,如酶联免疫分析法(ELISA)。此外,近年来,随着世界各国对转基因生物安全问题的日益关注,还涌现出了一批新的检测方法,如微阵列分析法(microarray),色谱分析法(chroma-tography),表面等离子共振(surfaceplasmonresonance,SPR)生物传感器分析法以及近红外线光谱分析法(nearinfraredspectroscopy,NIR)等。将对各种转基因检测方法的原理、特点及研究现状做一个扼要介绍。
Detection of genetically modified organisms（OMOs）is current issue that is gaining worldwide interest due to the ever-increaslng global diffusion and the related socio-economical implications. Current methodologies for the analysis of genetically modified organisms can be classified into two ways：DNA-based analytical methods,e, g. polymerase chain reaction, and proteln-based analytical methods, e. g. enzyme linked immunosorbent analysis. Recent years, new methodologies are developed, including microarrays, chromatography, near infrared spectroscopy and surface plasmon resonance biosensor. This review will give a summary to the status of the most widely used GMOs analysis technologies.
出处
《广西植物》
CAS
CSCD
北大核心
2006年第5期483-487,共5页
Guihaia
基金
国家转基因植物研究与产业化专项(J2002-B-005)~~
关键词
转基因产品
检测
方法
genetically modified organisms
detection
methodology
