托吡酯对戊四氮点燃慢性癫大鼠海马神经细胞黏附分子的影响

Effect of Topiramate on Brain Hippocampus Plasticity of Neurons in Pentylenetetrazol Induced Chronic Epilepsia Rats

目的:探讨神经元活化在癫发生、发展中的作用及托吡酯对其的影响。方法:采用戊四氮制备慢性癫模型,利用托吡酯干扰,选取不同时间点,观察大鼠行为学变化及神经细胞黏附分子在海马回的表达(免疫组织化学染色)。结果:托吡酯组点燃率在27d明显低于模型组;模型组及托吡酯组神经细胞黏附分子表达增加,并随时间延长明显;而不同时间点该表达的增加程度,托吡酯组均低于模型组。结论:神经细胞黏附分子表达的增加,说明出现了神经元活化及脑可塑性变化,而托吡酯明显地抑制了该表达的增加。

Aim： To Study the effect of brain hippocampus plasticity of neurons in the process of occurrence and development of epilepsy and the effect of topiramate （TPM）on behavior and brain hippocampus plasticity of neurons. Methods： Rats were randomly divided into 3 groups, control group（ C group）, PTZ group （P group, P1-10 d, P2-20 d and P3-27 d）and TPM＋PTZ group（TP group,TP1-10 d, TP2-20 d and TP3-27 d）.The behavior and expression of neural cell adhesion molecule （NCAM） by immunohistochemical staining were observed for 10, 20,27 days after using PTZ. Results： Significant difference in behavior between group P and TP after 4 weeks. Expression of NCAM increased in group P and TP （P〈0.05）, and continuously increased along with time, and the expression in P group is more higher than in group TP at different time-points （P〈0.05）.Condusion： The increasing of expression of NCAM in hippocampus indicated that plasticity of neurons had occurred and neural plasticity may be an important mechanism of epileptogenesis in the process of PTZ kindling rats. Expression of NCAM was inhibited markedly by TPM, indicating that TPM can influence expression of NCAM and may influence nerve plasticity.