摘要
目的研究葛根素对培养脑细胞缺氧/再给氧损伤的保护作用及其可能的机制。方法复制培养脑细胞缺氧/再给氧损伤模型,缺氧60min,再给氧30min。观察葛根素对脑细胞超微结构的影响。以ACAS570进行细胞内游离钙的定性及半定量检测;以荧光染料Fura-2-AM定量测定细胞内游离Ca2+浓度;以荧光偏振法测定细胞膜脂质流动性。结果葛根素能减轻脑细胞线粒体超微结构的损伤;可剂量依赖性地降低[Ca2+]i,提高细胞膜脂质流动性;可明显减少Ca2+伪彩色三维色彩值。结论葛根素对培养脑细胞缺氧/再给氧损伤有明显保护作用,可能与其降低[Ca2+]i和提高细胞膜脂质流动性有关。
Aim To study the protective effect of puerarin （PUE） on cultured cerebral cells injured by anoxia-reoxygenation. Methods The anoxia-reoxygenation injury model were developed, anoxia for 60 min and reoxygenation for 30 min. the effect of PUE on cerebral uhrastructure was observed. [Ca^2＋]i was estimated with Adherent Cell Analysis and Sorting 570 （ACAS 570） Laser Cytometer and measured with fluorescent dye Fura-2-AM, the lipid fluidity of cellular membrane was determined by fluorescence polarization technique. Results PUE improved the ultra-structure of cerebral cells, dose-dependently decreased [Ca^2＋]i and increased the lipid fluidity of cellular membrane. PUE markedly reduced the chromaticity value of pseudo-colour graphic model of Ca^2＋. Conclusion Puerarin has protective effect on anoxia-reoxygenation induced cerebral cell injury. The effect may be mediated by decreasing [Ca^2＋]i and increasing the lipid fluidity of cellular membrane.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第9期1130-1133,共4页
Chinese Pharmacological Bulletin
基金
大庆石油管理局科研资助课题(No0111005)
