大明胶囊对大鼠心肌梗死预防性保护作用的研究

Protective effects of Daming capsule preconditioning on myocardial infarction during regional ischemia in adult rats

目的观察大明胶囊对大鼠心肌梗死的预防性保护作用。方法对大鼠预防性给予大明胶囊1wk后,结扎其左冠脉前降支造成心肌梗死。通过测量心肌梗死面积,观察心肌酶学指标、病理形态学和组织超微结构的改变,评价大明胶囊对心肌梗死的预防性保护作用。结果心梗染色面积和心肌酶学指标显示(与模型组相比),大明胶囊灌胃后心肌梗死面积减少(P<0.05),乳酸脱氢酶(LDH)和肌酸激酶(CK)的含量降低(P<0.05)。心肌形态学和超微结构显示,大明胶囊减轻了左冠脉前降支结扎所造成的心肌损伤。结论通过心梗面积、心肌酶学指标、形态学和超微结构的观察,大明胶囊能预防性保护心肌梗死所造成的损伤。这提示大明胶囊可能应用于心肌梗死治疗。

Aim To observe protective effects of Daming capsule preconditioning on myocardial infarction during regional ischemia in adult rats. Methods Daming capsule was administered to rats for a week. After that, myocardial infarction was induced by occlusion of the left anterior descending coronary artery. Through observation of the myocardial infarct size, myocardial enzymes, morphology and uhrastructural organization of myocardium, protective effects of Daming capsule preconditioning on rat myocardium were evaluated. Resuits Compared with model group, contents of myocardial infarct size, lactic acid dehydrogenase （LDH） and creatine kinase （CK） in adult rats were diminished after intragastric administration of Daming capsule （P 〈 0.05）. Through observation of myocardial morphology and ultrastructural organization, it could be concluded that the myocardium injury caused by occlusion of the left anterior descending coronary artery was attenuated by Daming capsule. Conclusion Through the examination of myocardial infarct size, myocardial enzymes, morphology and ultrastructural organization, it showed that Daming capsule had protective effects on myocardial infarction, and indicated that Daming capsule might be applied in the treatment of myocardial infarction.