不同生物碱对小鼠S_(180)肉瘤细胞获得性多药耐药相关生物因子表达的干预

Interfere in Correlated Molecular Mechanism Obtained Multi-drug Resistance of Mouse S_(180)'s Tumour Cell for Different Alkaloid

目的:探讨中药生物碱口服给药对化疗诱导肿瘤MDR干预的分子生物学基础,以指导临床应用中药干预化疗所致MDR的产生。方法:以亚于治疗剂量的联合化疗,同时给予苦参碱、粉防己碱、氧化苦参碱和盐酸小檗碱4周,流式细胞仪荧光检测MDR肿瘤细胞P170、LRP、FOPOⅡ、Fas、CD54和细胞凋亡率。结果:苦参碱、粉防己碱明显降低化疗诱导后耐药细胞P170、LRP的表达率和TOPOⅡ活性,增加凋亡基因Fas表达率,减少细胞黏附因子CD54的表达,促进耐药肿瘤细胞的凋亡;而氧化苦参碱和盐酸小檗碱作用不明显。结论:苦参碱、粉防己碱可通过对MDR相关生物活性物质的调节,干预化疗诱发MDR的产生,并且其作用程度与生物碱的结构相关。

Objective： Observe the base of the interfere in correlated biotic active matter obtained multidrugresistance of induced by chemotherapy for different alkaloid. And then supervise the use of TCM in clinic torestrain the multi-drug resistant of chemotherapy, thereby improve the curative effect of mechanism. Methods： by the methods of less dosage of chemotherapy PFC, set up the mouse models of multi-drug resistance of S180 tumour cell. At the same time give the mouse matrine, terandrine, oxymatrine and berberine hydrooh loride 4weeks and then observe the P170, LRP, TOPOⅡ, Fas, CD54 and apoposis by flow cytometry. Results： Matrineterandrine can obviously reduce the express of P170, LRP, the activation of TOPO Ⅱ and increase the ratio ofthe express of Fas and the apoposis of drug resistant turnout cell. And at the same time it can obviously reducethe express of intercellular adhesion molecule （CD54）. Conclusion： Alkaloid of CTM, with the its adjustment of correlated biotic active matter,can intervene the occurrence of the muhi-drug resistance of tumor cellsinduced by chemotherapy, besides the different, degree of alkaloid of CTM＇ s effect with different configuration.