TGF-β_1、p15在食管鳞癌中的表达及其生物学意义

Expression and biological significance of TGF-β_1,p15 in esophageal squamous carcinoma

目的探讨转化生长因子1β(TGF-1β)、p15基因及其蛋白在食管鳞癌中的表达与食管鳞癌发生的关系。方法采用免疫组化SP法和原位杂交技术检测48例食管鳞癌组织,18例癌旁不典型增生组织和10例切缘正常组织中TGF-1β与p15 mRNA及其蛋白的表达。结果食管鳞癌组织中TGF-1β的阳性表达率(62.5%)低于正常组织(100%),P<0.05;食管鳞癌组织中p15 mRNA和p15蛋白的阳性表达率分别为56.3%和47.9%,二者表达明显相关(χ2=22,P<0.05),均低于在正常组织和不典型增生组织中的表达(P<0.05);食管鳞癌组织中TGF-1β的表达与p15 mRNA的表达明显相关(χ2=23.84,P<0.05)。结论食管鳞癌组织中TGF-1β对p15基因及其蛋白的转录可能有正向调节作用;TGF-1β、p15基因及其蛋白的低表达可能与食管鳞癌的发生有关。

[-Objective] To investigate transforming growth factor-β1 （TGF-β1）and p15 expression in the esophageal squamous carcinoma and the putative roles in carcinogenesis of esophageal squamous carcinoma. [Methods] The expressions of TGF-β1 protein, p15 protein and p15 mRNA were examined by SP immunohistoehemistry （IHC） method and in suit hybridyzation （ISH） in 48 eases of esophageal squamous carcinoma, 18 eases of esophageal dysplasia and 10 eases of ineisal margin normal tissues. [Results] The positive expression rate of TGF-β1 in esophageal carcinoma （62.5 %） was lower than that of normal tissues （100 %）; The positive expression rates of p15 mRNA and p15 protein in esophageal carcinoma were 56.3 % and 47.9 %, which were respectively lower than those in ineisal margin normal tissues and adjacent dysplasia tissues （ P〈 0. 05）. There was marked correlation between the expression of p15 mRNA and p15 protein in esophageal carcinoma tissues （X^2= 22, P 〈0.05）. There had marked relation between the expression of TGF-β1 and p15 mRNA in esophageal carcinoma tissues （X^2=23.84, P 〈0.05）. [-Conelusions-] TGF-β1 may play positive roles in p15 transcription. The low expressions of p15 and TGF-β1 might involve in esophageal carcinogenesis.