司帕沙星在E.coli-MG混合感染鸡的药动学与生物利用度研究

Pharmacokinetics and Bioavailability of Sparfloxacin in Experimentally Induced E.coli-Mycoplasma Ill Chickens

大肠杆菌-败血霉形体(Escherichia Coli.-Mycoplasma gallisepticu,E.coli-MG)疾病模型鸡单剂量(5mg.kg-1)内服和静注司帕沙星,研究其血液动力学特征和内服生物利用度。采用HPLC面积-内标法测定血浆中司帕沙星浓度,利用药动学分析软件MCPKP分析药-时数据。结果表明:疾病模型鸡内服司帕沙星血浆药-时数据符合一级吸收二室开放式模型,主要动力学参数如下:t1/2α1.7208h,t1/2β13.1773h,tm ax0.9083h,Cm ax0.6198μg.mL-1,AUC 3.8161mg.L-1.h-1。静注给药血浆中司帕沙星的经时数据符合无吸收二室开放式模型,主要动力学参数为:t1/2α0.4442h,t1/2β4.7557h,Kel0.5608h-1,Vd4.1204L.Kg-1,AUC 8.3274mg.L-1.h-1,C lB0.6004L.Kg-1.h-1。疾病模型鸡内服司帕沙星的生物利用度为47.03%。

A single dose （5mg· kg^-1） of sparfloxacin was given to experimentally induced E. coli-MG chickens by oral and intravenous injection. The drug concentration in plasma was detected with HPLC and the pharmacokinetics parameters were analyzed with MCPKP. The results showed that the blood concentratlon-time curves after oral administration were best described by a two compartment open model with first-order absorption rate. The main pharmacokinetic parameters were as follows： t1/2α 1. 7208h, t1/2β13. 1773h, tmax 0. 9083h, Cmax 0. 6198（ g· mL^-1 , AUC 3. 9161mg · L^-1 · h^-1. After intravenous administration, the plasma concentration-time data of sparfloxaein in ill chickens was fitted to a two compartment open model without absorption, and the main pharmacokinetic parameters were as follows： t1/2 α0.4442h, t1/2β4.7557h, AUC 8.3274mg· L-1 · h^-1. Kel 0.5608h^-1, Vd4.1204L · Kg^-1, AUC 8.3274mg · L^-1· h^-1, ClB 0. 6004L · Kg^-1· h^-1 The oral bioavailability of sparfloxaein in the ill model ehiekens was 47. 03%.