水杉总黄酮对胰岛素样生长因子-Ⅰ诱导的乳鼠心肌细胞肥大的抑制作用

Effects of Total Flavone of Metasequoia Glyptostroboides on the Proliferation of Rat Cardiac Myocardial Cell

目的探讨水杉总黄酮(TFM)对胰岛素样生长因子-Ⅰ(IGF-Ⅰ)诱导的体外培养的乳鼠心肌细胞DNA和RNA合成的影响。方法体外分离培养乳鼠心肌细胞,分成4组,即正常对照组(C组)、IGF-Ⅰ组、IGF-Ⅰ+TFM组、IGF-Ⅰ+月见草油(OBL)阳性对照组,采用细胞CT扫描定量分析心肌细胞的DNA、RNA含量。结果IGF-Ⅰ组心肌细胞的DNA和RNA合成明显增加,与正常对照组比较,均有显著性差异(P<0.05);IGF-Ⅰ+TFM组心肌细胞的DNA和RNA合成明显低于IGF-Ⅰ组,两组比较差异明显(P<0.05),与C组接近(P>0.05)。结论水杉总黄酮对IGF-Ⅰ诱导的乳鼠心肌细胞的肥大具有抑制作用。

Objective：To investigate the effect of total Flavone of Metasequoia Mlyptostr-oboides on synthesizing of DNA and RNA of rat myocardial cells （ MCs）. Methods： Isolated and cultured MCs of neonatal Sprague-Dawley （SD） were divided in to four groups, that is contral, insulin-like growth factor-Ⅰ （ IGF-Ⅰ）, IGF-Ⅰ＋ Total Flavone of Metasequoia Glyptostroboides（TFM） and IGF-Ⅰ＋ Oenothera Biennis L oil（OBL） group. The DNA and RNA of MCs of all groups were measured by cell Computor tolo after incubation. Results： Comparing with the contral groups the content of DNA and RNA of MCs in IGFs group was siglificently increased （ P 〈 0.05）, but the DNA and RNA of MCs in IGF-Ⅰ＋ TFM and IGF-Ⅰ＋ OBL groups obviously decreased（ P 〈 0.05） and close to C group, Conclusion： TFM effectively inhibits the proliferation of cultured MCs in rat by IGF- I inducing, which may play a role in the regression of heart remodeling.