乌梅丸治疗糖尿病的拆方研究

Study on Separated Recipes of Dark Plum Pill in Treating Diabetes

目的以“消渴从肝论治”立论,以乌梅丸作为治疗糖尿病的基础方,从实验研究力面,探讨乌梅丸的组方规律及其在糖尿病治疗中的作用和机制。方法取SD大鼠腹腔注射四氧嘧啶,建立类Ⅰ型糖尿病大鼠模型,然后随机分乌梅丸全方组、酸味组、苦味组、甘味组、辛味组和消渴丸组、模型组,并设空白对照组,给药2周后摘眼球取血,检测血糖、血胰岛素及血清甘油三脂和总胆固醇,并剖取胰腺组织制备病理切片以观察药物对胰岛的影响。结果各拆方组中,苦昧组血糖值降低最为明显,辛味组在一般状况的改善和血清甘油三脂和总胆固醇的降低作用方面效果最为明显。而在降血糖、降血脂、改善一般状况的综合作用上,全方组优于其它各拆方组,效果最佳。结论①乌梅丸具有降血糖作用,其作用机理可能是促进胰岛β-细胞再生和功能恢复、刺激胰岛β-细胞分泌胰岛素、增加外周组织对葡萄糖的利用等多方面的综合作用而实现的。②乌梅丸对于糖尿病大鼠具有良好的改善一般状况的作用。③乌梅丸方各组分中,苦味的黄连、黄柏具有较好的降血糖作用,辛味的附子、桂枝、细辛、川椒等具有较好的降血脂和改善一般情况的作用。

Subject： Based on the theory of treating polydipsia in diabetes through adjusting liver＇ s function, choose Dark plum pill（DPP）as the basic recipe of treating polydipsia in diabetes,and study the effect and mechanism in experiment. Method：Take SD rats and set up hyperglycemia models induced by alloxan. Then rats were randomly divided into seven groups ： DPP group, pathology group, sour flavour（SF） group, bitter flavour（BF） group, sweet flavour（SF） group, pungent flavour（PF） group and Xiaoke Wan （ XKW ） group, and take another 12 normal rats as normal group. After two weeks of treatment, rats were examined via routine methods for the measurements of the fasting blood sugar, insulin, triglyceride, cholesterol,and to take the pathological examination of the pancreas. Result： Among separated recipes groups, the BF group＇s BS decreased most evidently,and so did the PF group in blood lipid. But on the comprehensive effect of BS,blood lipid and general state, DPP group was better than other groups evidently. Conclusion： DPP can decrease blood sugar, blood lipid and ameliorate general state. The possible mechanism is as follows： DPP may facilitate the regeneration of pancreatic island β-cell and the restore of β-cell function,stimulate excretion of the β-cell,increase the sugar utilization of peripheral tissue and increase the number of glycogen in liver.