久效磷对单环刺螠体内几种酶活性影响的初步研究

The effects of monocrotophos on several enzyme activities in Urechis unicinctus

运用冰冻切片技术和酶组织化学的方法研究了长期暴露于不同质量浓度久效磷(0,50,100,150ng/L)下的单环刺螠(Urechisunicinctus)体内的乙酰胆碱酯酶(AChE)、细胞色素C氧化酶(CCO)、碱性磷酸酶(AKP)的活性变化。实验表明,AChE在单环刺螠的体壁上呈现出较强的酶活性,活性位点处为红棕色的颗粒沉淀、并随久效磷胁迫浓度的提高显色越来越深;消化道切片上基本上没有AChE的活性。CCO在单环刺螠的体壁及肠道上都呈现出较强的酶活性,活性位点显示为棕色;肠道上,不同质量浓度组由低到高其CCO活性位点依次增多、显色也依次加深。低质量浓度久效磷(<150ng/L)胁迫对单环刺螠体内的AKP活性没有明显的影响。

In this paper, Urechis unicinctus was exposed to different concentrations of monocrotophos （0, 50, 100 150 ng/L） for 45 days. The technique of frozen section was used to examine the changes of activities of acetylcholinesterase （ACHE） and cytochrome C oxidase （CCO） and alkaline phosphatase （AKP）. Results revealed that monocrotophos stress could decrease the AChE activities in the muscle of U. unicinctus and it was found that the activity of CCO in digestive tract became more active when the concentration of monocrotophos was higher. No significant effects were observed on the activity of AKP exposed to 50, 100 and 150 ng/L monocrotophos.