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L. rhamnosus 719产酸与抑菌作用的关系研究 被引量:2

Study on Relationship Between the Organic Acid Produced by L. Rhamnosus 719 and Inhibitory Effects
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摘要 HPLC分析表明,L.rhamnosus719在MRS中发酵时,醋酸含量先升后降,而乳酸在发酵过程中不断增加,发酵过程中,乳酸和醋酸的摩尔浓度比从6h的0.28增加到60h的2.63。L.rhamnosus719在MRS培养48h的上清液抑菌效果最好,抑菌圈直径达36mm,其中乳酸、醋酸含量分别为17.70g/L和4.32g/L。L.rhamnosus719产生较高浓度的乳酸和醋酸是其抑菌作用的主要原因。 By means of HPLC, the concentration of acetic acid increased and then decreased, the concentration of lactic acid continued to increase during fermentation of L. rhamnosus 719 at MRS. During the whole fermentation, the molecular ratio between lactic acid and acetic acid increased from 0.28 at 6h to 2.63 at 60h. The culture supernatants after 48 b incubation were examined to have the best inhibitory effect, and the inhibitory diameter was 36 mm. The concentration of lactic acid and acetic acid in culture supernatants was 17.70 g/L, 4.32 g/L, respectively. The main inhibitory effect was caused by high level lactic acid and acetic acid produced by L. rhamnosus 719.
出处 《农产品加工(下)》 2006年第10期111-114,共4页 Farm Products Processing
基金 广东省科技攻关项目(2003C20408及2005B10401006)
关键词 干酪乳杆菌鼠李糖亚种 有机酸 抑菌作用 HPLC lactobacillus casei subsp, rhamnosus organic acid inhibitory effect HPLC
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  • 1马绪荣 苏德模.药品微生物学检验手册[M].北京:科学出版社,2001.106-121.
  • 2钱存柔 黄仪秀.微生物学实验教程[M].北京:北京大学出版社,2000.48-59.
  • 3ADAMS M R, NICOLAIDES L. Review of the sensitivity of different foodborne pathogens to fermentation [J]. Food Control, 1997, 8: 227-239.
  • 4ZUBILLAGA M, WEILL R, POSTAIRE E, et al. Effect of probiotics and functional foods and their use in different diseases [J]. Nutrition Research, 2001, 21: 569-579.
  • 5SONGISEPP E, KULLIAAR T, HuTT P, et al. A new probiotic cheese with antioxidative and antimicrobial activity [J]. Journal of Dairy Science, 2004, 87:2017-2023.
  • 6KALTA A-K, KRUSE H, JOHNSEN G, et al. Antimicrobial susceptibility of starter culture bacteria used in Norwegian dairy products [J]. International journal of food microbiology, 2001, 67:147-152.
  • 7BOUKSAIM M, LACROIX C, AUDET P, et al. Effects of mixed starter composition on nisin Z production by Lactococcus lactic subsp.lactis biovar, diacetylactis UL 719 during production and ripening of Gouda cheese [J]. International Journal of Food Microbiology, 2000,59: 141-156.
  • 8HOTZAPFEL W, GEISEN R, SCHILLINGER U. Biological preservation of foods with reference to protective cultures, bacteriocins and food-grade enzymes [J]. International Journal of Food Microbiology, 1994, 24: 343-362.
  • 9PRASAD J, GILL H, SMART J, et al. Selection and characterisation of Lactobacillus and Bifidobacterium strains for use as probiotics [J].International Dairy Journal, 1999, 8: 993-1002.
  • 10WANG Y C, YU R C, YANG H Y, et al. Sugar and acid contents in soymilk fermented with lactic acid bacteria alone or simultaneously with Bifidobacteria [J]. Food microbiology, 2003, 20:333-338.

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  • 1刘冬梅,李理,杨晓泉,梁世中.用牛津杯法测定益生菌的抑菌活力[J].食品研究与开发,2006,27(3):110-111. 被引量:275
  • 2刘冬梅,李理,梁世中,杨晓泉.潜在益生菌鼠李糖乳杆菌在大豆奶酪中的稳定性[J].华南理工大学学报(自然科学版),2006,34(5):76-80. 被引量:9
  • 3GUINANE C M, COTTER P D, HILL C, et al. Microbial solutions to microbial problems: lactococcal bacteriocins for the control of undesirable biota in food [J]. Journal of Applied Microbiology, 2005, 98:1316-1325.
  • 4LUECKE F K, HECHELMANN H. Starter cultures for dry sausages and raw ham: composition and effect[J]. Fleischwirtschaft, 1987, 67:307-314.
  • 5FORESTIER C, CHAMPS C D, VATOUX C, et al. Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties [J]. Research in Microbiology, 2001, 152: 167-173.
  • 6De CHAMPS C, MARONCLE N, BARESTRINO D, et al. Persistence of colonization of intestinal mucosa by a probiotic strain, Lactobacillus casei subsp, rhamnosus Lcr35, after oral consumption [J]. Journal of Clinical Microbiology, 2003, 41(3): 1270-1273.
  • 7RAY B, DAESCHEL M. Food biopreservatives of microbial origin [M]. Florida: CRC Press, 1992.
  • 8SCHILLINGER U, LUCKE F K. Antibacterial activity of Lactobacillus sake isolated from meat [J]. Applied and Environmental Microbiology, 1989, 55: 1901-1906.
  • 9BAREFOOT S F, KLAENHAMMER T R. Detection and activity of lactacin B, and bacteriocin produced by Lactobacillus acidophilus[J]. Applied and Environmental Microbiology, 1983, 45: 1808-1814.
  • 10JUNG D S, BODYFELT F W, DAESCHEL M A. Influence of fat and emulsifiers on the efficacy of nisin in inhibiting Listeria monocytogenes in fluid milk [J]. Journal of Dairy Science, 1992, 75: 387-392.

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