摘要
在雨生红球藻培养液中分别添加活性氧1O2、H2O2和·OH的诱生剂,通过测定细胞密度、叶绿素a含量、虾青素含量,研究了这三种活性氧诱生处理对雨生红球藻生长和虾青素含量的影响,初步探索了利用活性氧诱生剂提高雨生红球藻虾青素含量的可行性。实验结果表明,适当浓度的MB能够促进虾青素含量增加,当MB浓度为10-7mol·L-1时,虾青素含量达到5.27μg·mL-1,比对照显著提高。活性氧诱生剂对雨生红球藻生长有抑制作用,但MB的抑制作用小于H2O2和·OH诱生剂。
By determining the cell quantity, content of chlorophyll-a, astaxanthin content in Haematococcus pluvialis, the effects of three reactive oxygen species ^1O2, H2O2 and -OH on the growth and the accumulation of astaxanthin content were investigated,, preliminarily seek to the feasibility to enhance the astaxanthin biosynthesis by ROS. The results showed that 1） the content of asataxanthin in Haematococcus pluvialis was obviously increased by proper concentration of MB. When the concentration of MB was 10^-7 mol ·L^-1, content of asataxanthin could reach to 5.27μg ·mL^-1, much higher than the control. 2） the growth of Haematococcus pluvialis was restrained by the ROS, but the restraining effect of MB was not so serious as H2O2 and reagent of·OH.
出处
《生态科学》
CSCD
2006年第3期213-215,221,共4页
Ecological Science
基金
国家自然科学基金(30370231)
珠海市科技计划重大项目(PB20041018)
广东省科技计划重点引导项目(2005B33201001)
关键词
雨生红球藻
活性氧
虾青素
Haematococcus pluvialis
Reactive oxygen species（ROS）
Astaxanthin
