凋亡抑制基因survivin在膀胱癌组织中的表达

The expression of antiapoptosis survivin in bladder carcinoma

目的探讨新的凋亡抑制基因survivin在膀胱癌组织中的表达及其与膀胱癌生物学特性的相关性。方法应用免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法(SP),检测survivin在42例膀胱癌组织、8例正常膀胱组织中的表达。结果Survivin在正常膀胱组织中不表达;42例膀胱癌组织有28例表达阳性,阳性率为66.7%。病理分级Ⅰ-Ⅲ级膀胱癌survivin阳性表达率分别为33.3%、76.2%、88.9%,差别有统计学意义(P<0.05);临床分期T1-T3期膀胱癌的阳性表达率分别为60.9%(14/23)、66.7%(8/12)、85.7%(6/7),差别无统计学意义(P>0.05);无复发与有复发膀胱癌的阳性表达率分别为53.8%(14/26)、87.5%(14/16),差别有统计学意义(P<0.05)。结论Survivin阳性表达引起的细胞凋亡抑制在膀胱癌的发生、发展中起一定作用,其过度表达可能提示膀胱癌预后不良。

Objective To study the expression of a novel inhibitor of apoptosis, survivin, in bladder carcinoma and its relationship with biological characteristic of bladder carcinoma. Methods Using streptavidin-biotin peroxidase （SP） method, we examined the expression of survivin in 8 normal vesical and 42 bladder carcinoma tissues, Results Survivin was expressed in 28 of the 42 （66.7%） cases of bladder carcinomas but was not expressed in normal bladder epithelium. The positive rate of survivin in grade I, grade II and grade III was 33.3%, 76.2% and 88.9% respectively, and the difference was significant （ P〈 0.01）. The positive rate of survivin in T1, T2, and T3 was 60.9%, 66, 7% and 85.7%, respectively, and the difference wasn＇t significant （P〉0.05）. The positive rate of survivin in recurrent and non-recurrent group was 53.8 % and 87,5 %, respectively, and the difference was significant （P〈0.01）. Conclusion Apoptosis inhibited by survivin may participate in the onset and progression, The overexpression of survivin may suggest the poor prognosis of bladder carcinoma.