膀胱移行细胞癌骨桥蛋白的表达及其临床意义

Expression of osteopontin protein in transitional cell carcinomas of the bladder and its clinical significance

目的探讨骨桥蛋白(osteopontin,OPN)在膀胱移行细胞癌(transitionalcellcarciromas,TCC)组织中的表达及其与肿瘤发生、发展及临床预后的联系。方法免疫组织化学SABC法检测60例膀胱移行细胞癌、20例正常膀胱壁标本的OPN表达强度,分析OPN表达与肿瘤分期、分级、复发、转移及患者5年生存率之间的关系。结果在60例膀胱TCC细胞浆OPN强染色(OPN评分4-8分):T1和T216例(43.2%),T3和T420例(86.9%),Ⅰ级5例(38.4%),Ⅱ级6例(35.2%),Ⅲ级25例(83.3%),复发患者21例(87.5%),初发患者15例(41.7%),淋巴结或远处转移阳性患者17例(80.9%),阴性19例(48.7%),男性患者31例(75.6%),女性患者5例(26.3%)。OPN强染色预示患者较高的复发率和较短的生存时间。结论OPN表达强度与肿瘤分期、分级、复发、转移、5年生存率及性别密切相关,说明OPN参与肿瘤的发生、发展,并对肿瘤患者的预后起重要作用,且OPN的表达有一定的性别差异。

Objective To investigate the expression of osteopontin （OPN） in transitional cell carcinomas of the bladder and the significance of OPN in tumorigenesis and prognosis. Methods Immunohistochemistry has been used to detect OPN in 60 transitional cell carcinomas of the bladder and 20 normal tissues. The correlation of OPN and tumor stage, grade, recurrence, metastatic,survival rate, and gender of patients were analyzed. Results High cytoplasmic OPN staining was observed in 43.2% of tumor stage T1 andT2, 86.9% of T3 and T4, 38.4% of tumor grade Ⅰ , 35.2% of grade Ⅱ , 83.3% of grade Ⅲ, 87.5% of recurrence tumors, 80.9% of metastatic cases, 75.6% of male patients, 26.3% of female patients. High OPN expression portent showed a high risk of recurrence and short survival time of patients. Conclusion This study reports the OPN protein expression in transitional cell carcinomas of the bladder, suggesting involvement of this protein in tumor formation. The strong correlation between pathological stage, tumor grade, recurrence, metastatic, survival rate and OPN suggests a role for OPN in tumor progression. The degree of OPN expression is higher in males than females.