API_（0134）对犬心肌缺血－再灌注过程中血浆6－酮－前列腺素F_（1α）和粒细胞产生氧自由基的影响

Effects of API 0134 on Plasma 6 keto PGF 1α and Release of Oxygen Free Radicals by Neutrophils During Myocardial Ischemia/Reperfusion in Dogs

目的：研究药物ＡＰＩ０１３４对实验性急性心肌缺血—再灌注过程中血浆６－酮－前列腺素Ｆ１α和粒细胞产生氧自由基的影响。方法：结扎犬冠状动脉左前降支９０分钟后行再灌注１２０分钟。用药组（ｎ＝８）在冠状动脉左前降支结扎４５分钟时静脉给予ＡＰＩ０１３４（９ｍｇ／ｋｇ）至再灌注６０分钟。对照组（ｎ＝８）给予同等量５％葡萄糖盐水。放射免疫法测定血浆６－酮－前列腺素Ｆ１α和凝血烷Ｂ２（ＴＸＢ２，血栓素Ｂ２）含量，化学发光法测定粒细胞产生氧自由基的量。结果：与对照组比较，用药组在用药后心肌缺血—再灌注期间，血６－酮－前列腺素Ｆ１α增高（Ｐ＜０．０５）、凝血烷Ｂ２降低，粒细胞产生氧自由基的量减少（Ｐ＜０．０５）。结论：ＡＰＩ０１３４可促进心肌缺血—再灌注过程中６－酮－前列腺素Ｆ１α（即前列腺素）的生成，抑制粒细胞产生氧自由基，这可能是该药减轻心肌再灌注损伤的重要机制之一。

Objective:To study the effects of Andrographi Peniculata isolates 0134 ( API 0134 )on plasma 6 keto prostaglandin(PG)F 1α and the release of oxygen free radicals by neutrophils during experimental acute myocardial ischemia reperfusion. Methods:Ligation of the left anterior descending coronary artery (LAD) in dog lasted 90 min followed by 120 min reperfusion.In the treated group ( n =8),infusion of API 0134 (9 mg/kg) started at 45 min after LAD ligation,and lasted to 60 min after reperfusion.In the control group ( n =8),the same amount of 5% glucose saline was given at the same time as above.Plasma concentrations of 6 keto PGF 1α and thromboxane(TXB 2) were determined by radioimmunoassay.Oxygen free radicals generated by neutrophils was measured by chemiluminescence. Results:Compared with the control group,plasma 6 keto PGF 1α in the treated group was increased ( p <0 05),TXB 2 decreased,and neutrophil induced oxygen free radicals were inhibited( p <0 05),during ischemia reperfusion after administration of API 0134 . Conclusion: API 0134 may increase the production of plasma 6 keto PGF 1α ,inhibit neutrophil induced oxygen free radicals during myocardial ischemia and reperfusion,which may be one of the important mechanisms in alleviating myocardial reperfusion injury.