羟基喜树碱对培养兔晶状体上皮细胞的影响

THE EFFECT OF HCPT ON RABBIT LENS EPITHELIAL CELLS IN VITRO

目的研究羟基喜树碱(hydroxycamptothecine,HCPT)对培养兔晶状体上皮细胞(rabbitlens epithelial cells,RLECs)形态、增殖的影响及其机制,筛选防治后发性白内障的药物。方法首先进行RLECs体外培养,在第2、3代RLECs中加入HCPT,分别用光学显微镜和透射电镜观察细胞形态的变化。然后,在第2、3代RLECs中加入不同浓度的HCPT,观察HCPT对细胞增殖的影响。结果①HCPT对RLECs的形态具有显著的影响,透射电镜下主要表现为调亡细胞的形态改变。②HCPT能抑制细胞的增殖,其抑制作用随药物浓度增加、作用时间延长而增强。结论①HCPT通过凋亡机制引起体外培养RLECs的调亡。②HCPT能抑制体外培养RLECs的增殖,其抑制作用呈剂量依赖性和时间依赖性。③HCPT可能成为预防后发性白内障的药物。

Objective To study the effect of hydroxycamptothecine （HCPT） on the morphological changes, proliferation of rabbit lens epithelial cells （RLECs） and to evaluate the drugs used for posterior capsularopacification（PCO）after cataract extraction. Methods RLECs were cultured in vitro and then HCPT was added to the rabbit lens epithelial cells of second or third passage. Cells morphological changes were observed with light microscope and electron microscope. HCPT of different concentrations was added to the rabbit lens epithelial cells of second or third passage. The effect of HCPT on the proliferation was observed. Results HCPT could significantly affect the forms of RLECs. Apoptosis was discovered with electron microscope. HCPT could inhibit the proliferation of RLECs and the number of cells with apoptosis increased with the HCPT concentrations and time. Conclusion HCPT can induce apoptosis of rabbit lens epithelial cells cultured in vitro. The number of cells with apoptosis increases with HCPT concentrations and time. HCPT might be a candidate to prevent the posterior capsularopacification.