摘要
培养杜仲愈伤组织并测定其含胶量,结果发现,杜仲胶的合成与愈伤组织的生长周期有一定的相关性。在继代培养中,随着继代次数的增加,含胶量呈增加趋势。外植体上愈伤组织一旦形成就有杜仲胶的积累,杜仲胶含量在S5~S8代出现一个高峰值,S8代愈伤组织的含胶量已经达到原植株的94.38%,S9代后又迅速下降。
This paper reported the dynamic change of gutta-percha content during callus culture of Eucommia ulmoides Oliv. The results showed that there was a relative close relationship existing between the synthesis of gutta-percha and the growth period of calluses. In callus subculture, the gutta content increased with the times of callus subculture. Once callus was formed, the guttapercha started to accumulate. The gutta-percha content has a peak value from subculture generation 5 to generation 8;it could reach 94.38 % of the original plant in subculture generation 8, After generation 9, it declined dramatically.
出处
《林业科技开发》
2006年第5期47-49,共3页
China Forestry Science and Technology
基金
陕西科技大学博士基金(项目编号:BJ04-03)
关键词
杜仲
愈伤组织
胶含量
Eucommia ulmoides Oliv,
Gutta-percha
Callus
Gutta-percha content