中晚期肺癌患者外周血CD4^+细胞因子水平的观察

CD4^+ cell cytokine level in mid-advanced lung cancer

目的观察中晚期肺癌患者CD4+细胞Th1、Th2因子的表达,探讨其在肺癌发病及分型中的意义。方法采取肺癌患者和健康体检者静脉血标本,用Th1、Th2细胞因子标记流式细胞技术检测IFN-γ、TNFα-、IL-2、IL-4、IL-6、IL-10,比较肺癌患者相对于健康体检者外周血CD4+细胞分泌细胞因子水平的变化。结果中晚期肺癌患者外周血CD4+细胞表达IFN-γ、TNF-α、IL-2水平均低于正常人(P<0.01);IL-4、IL-6、IL-10水平均高于正常人(P<0.01);中晚期小细胞癌、鳞癌、腺癌患者外周血CD4+细胞表达IFNγ-、TNFα-、IL-2、IL-4、IL-6、IL-10各组间无差异(P>0.05);中晚期肺癌患者外周血Th1/Th2(γ/4)低于正常人(P<0.01)。结论肺癌患者体内以Th2型细胞占优势状态,不同病理类型的肺癌Th1、Th2细胞因子间无显著性差异,血清与Th1细胞表达的TNFα-的比值可能对判断预后有一定意义。

Objective To explore the feature of Thl ,Th2 cytokine in mid-advanced lung cancer and their significance in morbidity and prognosis for lung cancer. Methocl IFN-γ,TNF-α, IL-2,IL-4,IL-6 and IL-10 in venous blood from patients with lung cancer and the healthy subjects were detected by flow cytometry marked by Thl and Th2 cytokines and the difference of CD4^＋ cytokine of both was compared. Results IFN-γ,IL-2,TNF-α and Th1/Th2 ratio（IFN-γ/IL-4）were obviously lower in lung cancer patients than in healthy subjects （P 〈 0.01 ）, while IL-4, IL-6 and IL-10 were significantly higher in lung cancer patients than in healthy subjects（P〈0.01 ）. There was no difference in SC, AD and SCLC. Conclusion Th2 is in majority in lung cancer. TNF-α（serum）/ TNF-α（Thl） ratio may have some significance to judge the prognosis.