HPLC法测定银菊散结口服液中绿原酸含量

Determination of chlorogenic acid in honeysuckle flower oral solution by HPLC

目的 建立高效液相色谱法测定银菊散结口服液中绿原酸含量的方法。方法 采用HPLC法，Kromasil C15（250mm×4.6mm，5μm）色谱柱，乙腈-0．4％磷酸（10：90）为流动相；检测波长为327nm。理论板数按绿原酸峰计算应不低于1000。流速：1ml·min^-１。结果 绿原酸线性范围为0～．05mg·ml^-1（r=0．9994），回收率（n=6）为102．2%，RSD=0.98％。结论 本方法分离效果好，准确可靠，是控制银菊散结口服液内在质量的有效方法。

OBJECTIVE To establish an HPLC method for the determination of chlorogenic acid in honeysuckle flower oral solution. METHODS The chromatographic condition included Kromasil analysis column （4.6mm～250mm, 5μm）and mobile phase of acetonitrile-0.4% phosphoric acid solution （ 10 ： 90） ;The detectoin wave-length was 327 nm. RESULTS The calibration curve was linear in range from 0～0.05mg·ml^-1（ r=0. 9994） ,the average recovery was 102.2% （n=6）,RSD = 0.98%. CONCLUSION The method was proved to be simple, rapid and accurate for the quality control of honeysuckle and chrysanthemum flower oral solution.