山仙颗粒对Lewis肺癌瘤株转染C57BL/6小鼠NK细胞和IL-1活性影响实验研究

Experimental Study on Effect of SXG on Activity of IL-1 and NK of C57BL/6 Mice with Lewis Lung Cancer of Spontaneous Metastasis.

目的探究山仙颗粒(SXG)对Lewis肺癌瘤株转染的C57BL/6小鼠NK细胞和IL-1活性的影响。方法将40只C57BL/6小鼠随机取出10只作为空白对照组(A组),把LEWIS肺癌细胞接种于剩余30只小鼠右腋皮下,再将它们随机分为荷瘤对照组(B组)、金龙胶囊组(C组)、山仙颗粒组(D组)等3组。接种24h后,A、B组用生理盐水灌胃。C、D组分别以金龙胶囊和山仙颗粒混悬液灌胃,造摸21天处死小鼠,检测移植瘤重、肺转移灶数、出瘤时间、NK细胞及IL-1活性。结果与对照组比较,SXG组小鼠出瘤时间显著延长(P<0·01),瘤重减轻、肺转移灶数减少、NK细胞及IL-1活性升高(P<0.01)。结论SXG能显著抑制LEWIS肺癌的生长与转移,其机制可能与提高IL-1及NK活性有关。

Objective To study the effect of Shan Xian Granule （SXG） on Lewis lung cancer of spontaneous metastasis, and activity of IL - 1 and NK in those mice. Methods 10 mice were selected from the 40 mice of C57BL/6 randomly as blank group （ A - group） , the rest 30mice were planted by Lweis lung cancer cell and they were divided randomly into Lweis lung cancer control group（ B -group）, Jinlong capsule group （C -group）,and SXG group （D - group）. After 24h they were planted, A,B groups mice were given normal saline, C,D groups were give JLC and SXG solution separately. Twenty - one days later, put those mice to death, then measured the time of the tumor coming into being, weight of the transplanted tumor, number of metastasis and the activity of IL - 1 and NK. Results the time of the tumor coming into being of SXG group was obviously longer than that of B - group. The weight of transplanted tumor was lighter than that of B - group. The activity of NK and IL - 1 was high - er than that of B - group. Conclusions SXG can inhibit the growth and metastasis of tumor. Its mechanism is the enhancement of the activity of NK and IL - 1.