肿瘤术后残留肿瘤细胞的DNA含量和增值指数的定量研究

Remaining Cancer Cells Research by Flow Cytometry after Tumor Surgery

目的探讨残留肿瘤细胞的生物学行为,为防治残留肿瘤细胞复发、延长患者远期生存提供理论依据。方法应用流式细胞术对各种病理类型的残留肿瘤细胞的DNA含量(D I)和增殖指数(PI)进行定量测定。结果(1)残癌的D I和PI明显低于主体癌(P<0.001);(2)阳性主体癌组的D I与阴性主体癌组比较无显著性差异(P>0.05),而两组PI差异有显著性意义(P<0.005);增生主体癌组与阴性主体癌组的D I和PI均无显著性差异(P>0.05);(3)癌中心和癌前缘组织的D I、PI均无显著性差异(P>0.05)。结论残留肿瘤细胞DNA含量和增值指数变化的生物学行为可作为临床防治残留肿瘤细胞复发、延长患者远期生存的理论依据。

Objective This thesis mainly aims at probing into the remaining tumor cells, so as to offer theoretical basis for preventing and curing the tumor cells for prolonging patients＇ long - term survivals. Methods Flow cytometry is applied to the quantitative research of the DNA content of various pathology＇s remaining tumor cells and proliferation index, so we can go a step further to do t test and chi- square test with the data we＇ve gotten. Results （1）The PI and DI that remains is obviously below the main cancer cells（P 〈0.001）;（2）The DI of the positive main cancer cell group doesn＇t have a significant discrepancy compared to that of the negative main cancer cell group（P 〉0.05）, But the discrepancy of the PI of the two groups do have that （P〈0. 005）;The PI and DI between the negative main cancer cell group and the hyperplasia cancer cell group have to significant difference（P 〉0. 05）;（3）The PI and DI between the center of the cancer cells and the leading edge of the cancer cells＇ organization have no significant discrepancy（P 〉0.05）. Conclusion There biology behaviors of the remaining tumor cells have offered theoretical basis for the clinical treatment and prevention.