摘要
将自测的瓢虫科4亚科16种和从GenBank中检索到相关物种的mtDNA-COI基因编码区序列片断进行同源性比较,计算核苷酸使用频率,并构建分子系统树。在获得的471bp序列中,共有227个变异位点,195个简约信息位点,A+T约占66.7%,转换发生比颠换更频繁。分子系统树表明:同种和同属的瓢虫均以较高置信值聚在一起,4个亚科都能恢复为单系;食植瓢虫亚科从系统树的基部最早分出,是瓢虫科最为原始的类群,小毛瓢虫亚科和盔唇瓢虫亚科首先聚为一支,显示二者具有较近的亲缘关系,然后再与瓢虫亚科相聚,食植瓢虫亚科的食植瓢虫属和裂臀瓢虫属均不是单系群。
Based on the mtDNA-COI gene of 16 species （belonging to 4 subfamilies of Coccinellidae） and relative species from GenBank, the homologus sequences were compared. The used frequency of nucleotide was calculated and the molecular phylogenetic tree was constructed. In the 471bp fragment of mtDNA-COI gene, there were 227 variable sites and 195 parsimony_informative sites, and A＋T was about 66.7%. The variation rate of transition was larger than transversion. The members of the same species or genus were grouped together with high bootstrap confidence values, and all the 4 subfamilies were monophyletic. Epilachninae was the most ancestral group in Coccinellidae. Scymninae was grouped with Chilocorinae first, showing they had close relationship, and then they were grouped with Coccinellinae. In Epilachninae, both Epilachna and Henosepilachna were not monophyletic.
基金
陕西省自然科学基金资助项目(97SM10)
