摘要
目的建立依达拉丰氯化钠注射液含量测定的HPLC方法。方法采用Hypersil C18柱（250mm×4．6mm，5μm），以甲醇-乙腈-0.1mol·L^-1 醋酸钠（40：15：55，冰醋酸调pH=4.5）为流动相，流量：1．0ml·min^-1，检测波长：245nm，进样体积：10出。结果线性范围为8．9—53．4μg·ml^-1（r=0．9998，n=6）；高、中、低3种不同浓度的平均回收率为99．50％（RSD％：0.35％）；精密度为0．220%（n=5）。结论本法快速、准确、专属性高，适于依达拉丰氯化钠注射液的含量测定。
AIM To develop a HPLC method for the determination of edaravone sodium chloride Injection. METHOD The determination was performed on a Hypersil C18 column（250 mm × 4.6 mm,5μm）with the mobile phase consisting of methanol-acetonitrile-0.1 mol·L^-1 sodium acetate buffer（40：15：55,regulate pH to4.5 with acetic acid） and detected at 245 nm. The flow rate was 1.0 ml·min^-1 The injection volume was 10/11. RESULTS The method offered good linearity from 8.9 to 53.4μg·ml^-1（ r = 0.9998, n =6）. The average recovery was 99.50% （ RSD % = 0.35 % ） for three different levels of the added amount of edaravone. The precision of the method was 0.22% （ n = 5）. CONCLUSION The developed HPLE method was,accurate and specific,and could be used for the detennination of edaravone in injection.
出处
《江苏药学与临床研究》
2006年第4期236-238,共3页
Jiangsu Pharmacertical and Clinical Research
