脑薄片实验中常见的问题及解决办法

Normal Issues in the Brain Slice Electrophysiological Experiment and the Ways to Dispoe it

在脑薄片实验中,实验是否成功,取决于脑脊液的配制、标本的制备、微电极的制作、干扰的排除以及仪器设备的质量和实验者的实验技术等各个环节,而保持脑薄片的生理活性及干扰的排除,是这些环节的关键。文中根据在脑薄片实验过程中遇到的常见问题,提出了解决的办法。

The concoct of the artificial cerebrospinal fluid, the sample of brain slice, the making of the glass micropipettes, elimination of the disturbance and the quality of the equips are all important key factors to the success of the rat brain slice electrophysiological experiment, and how to keep the physio- logical character of the brain slice and the elimination of the disturbance are the most important. In this article, we will discuss these factors and the ways to handle them.