VEGF、PCNA、MVD、flk-1在(人脑显形细胞瘤)中的表达及临床意义

The expression and its clinical significance of VEGF、PCNA、MVD and flk-1

目的研究血管内皮细胞生长因子(Vascularendothelialgrowthfactor,VEGF)及其受体flk1(fetalliverkinase1)在人脑显形细胞瘤中的表达与肿瘤增殖及血管再生的相互关系。方法应用免疫组化技术和形态定量分析法,检测69例手术切除脑胶质瘤中VEGF表达、PCNA标记指数(PCNALI)、微血管密度(Microvesseldensity,MVD)及部分病例flk1表达。结果(1)肿瘤细胞及血管内皮细胞均可以表达VEGF及flk1,阳性颗粒分布于肿瘤胞浆中。(2)高级别肿瘤PCNALI、MVD显著高于低级别肿瘤(P<0.05);VEGF表达阳性肿瘤的PCNALI、MVD显著高于VEGF表达阴性肿瘤(P<0.05);(3)VEGF与其受体flk1在肿瘤细胞中表达呈正相关;肿瘤组织VEGF及其受体flk1表达与肿瘤的恶性程度呈正相关;(4)在星形细胞肿瘤中,随着MVD的增大,VEGF及flk1在肿瘤血管内皮的染色率逐渐增加,分别与肿瘤的MVD存在正相关关系(r分别为0.40,0.44,P<0.01)。结论人脑显形细胞瘤可以合成VEGF,VEGF在肿瘤细胞增殖及血管再生过程中起重要作用。

Objective To investigate the relation between expression of vascular endothelia growth factor（VEGF） with it＇s receptor flk-1 and anglogenesis and cell proliferation in human gliomas. Methods Immunohistochemical technique and morphological quantitative analysis was used to detect the expression of VEGF, microvessel density（MVD） ,PCNA labelling index（PCNA LI） and the expression of flk- 1 labelling in the partial subgroup in 69 gliomas. Analyse the test result with a statistical method. Results （1） Positive staining for VEGF was in most gliomas. The tumor and vascular endothelial cell s both expressed VEGF and flk-1. The positive particle were most distributed in tumor cytoplasm. （2） In the astrocytic tumors,the higher the tumor grade,the stronger it expressed PCNA LI, the larger the MVD（ P 〈 0.05） ; the PCNA LI and MVD in VEGF positive tumors were significantly higher than those in VEGF negative tumors（ P 〈0.05）. （3）In the astrocytic tumors,it showed positive relativity between the expression of VEGF and flk- 1 in tumor cell.The higher the tumor grade,he stronger it expressed VEGF and flk-1（ P 〈0.01）.h showed positive relativity between the expression of VEGF with it＇s receptor flk-1 and tumor malignant degree. （4）In the astrocytic tumors,the expression of VEGF and flk-1 in vascular endothelial cells increased with the increase of MVD.It showed positive relativity between the expression of VFGF and flk- 1 in vascular endothelial cells to MVD（P 〈 0. 01 ）. Conclusion VEGF can be synthesized in tumor cells of humans glioma and it might play an important role in angiogenesis and cell proliferation.