人淋巴细胞免疫反应性生长激素mRNA的扩增与鉴定

Detecting Immunoreactive GH mRNA in Human Peripheral Blood Lymphocytes

用正常人淋巴细胞，总ＲＮＡ与人垂体生长激素特异性探针进行Ｎｏｒｔｈｅｒｎ杂交，得到一条弱的杂交带，其位置与人垂体生长激素分泌瘤总ＲＮＡ的Ｎｏｒｔｈｅｒｎ杂交带近乎一致。根据人垂体生长激素ｃＤＮＡ顺序设计合成寡核苷酸引物，用逆转录。聚合酶链反应相结合的方法，从正常人外周血淋巴细胞．总ＲＮＡ中扩增出的ｃＤ－ＮＡ与人垂体生长激素ｃＤＮＡ大小相似，证实正常人淋巴细胞有免疫反应性生长激素ｍＲＮＡ的表达。

The present study demonstrats that immunoreactive GH mRNA is expressed at low levelin norrnal human peripheral blood lymphocytes by RT-PCR amplification and hybridization. Northern blot analysis of total RNA from lymphocytes with a pituitary hGH cDNA probe re-vealed a faint transcript migrating almost coincident with pituitary hGH menssenger RNA. Reverse transcription polymerase chain reaction from total RNA of lymphocytes by usinghGH specific primers generated a predicted 702 base pair band with almost the same size aspituitary and also hybridized with pituitary hGH cDNA probe specifically in Southern blotanalysis,which indicates a low level expression of GH-like mRNA in human peripheral bloodlymphocytes.