醋酸棉酚对人射出精子Ca^（2＋）内流和大鼠输精管收缩反应的影响

Effects of Gossypol Acetic Acid on Human Ejaculated Sperm Ca2+ Influx and Rat Vas Deferens Contraction Action

本实验旨在观察醋酸棉酚对人射出精子Ｃａ^（２＋）跨膜转运和对离休大鼠输精管收缩作用的影响。结果表明，醋酸棉酚抑制人射出精子Ｃａ^（２＋）内流，并呈量－效依赖关系。醋酸棉酚破坏精子膜内、外Ｃａ^（２＋）浓度梯度的动态平衡，可能是其抑制精子活力的重要机理之一。但醋酸棉酚对高Ｋ＋诱发离体大鼠输精管平滑肌的收缩作用没有影响，可能醋酸棉酚对大鼠精子膜有选择性的作用。

It has been reported that gossypol acetic acid(GAA)inhibits sperm motility and calciumplays an important role in regulating the function of the sperm flagella.In order to explorethe mechanism by which GAA inhibits sperm motility,the effects of GAA on human ejacu-lated sperm Ca2+ influx and isolated rat vas deferens contraction action were studied.The re-suIts showed that GAA inhibited sperm Ca2+ influx in a dose-dependent manner,andbreaked the dynamic equilibrium of sperm internal and external Ca2+ gradient concentrations.It may be one of important mechanism by which GAA inhibits sperm motility.But GAA ex-erts no effect on the high K+ induced contraction of smooth muscle of isolated rat vas deferens,It is likely that GAA has selective action on the sperm membrane of the rats.