石榴皮对幽门螺杆菌的体外抑菌实验研究

Study of In Vitro Inhibition Effect of Pomegranate Rind on H.pylori

目的探讨石榴皮对幽门螺杆菌（H.pylori）甲硝唑耐药株及敏感株的体外抑制作用．方法琼脂稀释法检测H.priori甲硝唑耐药株和敏感株对石榴皮水煎剂的最低抑菌浓度（MIC），并计算MIC50、MIC75、MIC90．结果石榴皮对H.pylori的MIC值范围为7．8125-500mg／mL；石榴皮对H.pylori甲硝唑耐药株和敏感株的MIC50分别为29．9mg／mL和28．0mg／mL，MIC75分别为65．1mg／mL和59．1mg／mL，MIC90分别为131．1mg／mL和115．9mg／mL；H.prior／甲硝唑耐药株和敏感株对石榴皮水煎剂的敏感性无统计学差异（相对中位数潜力1．072，95％的可信区间为0．763—1．513）．结论石榴皮有良好的抑菌效果，H.prior／甲硝唑耐药株及敏感株都对其敏感．

Objective To discuss in vitro the inhibition effect of Pomegranate Rind on H. pylori metronidazole resistant strains and susceptible strains. Methods The Agar Dilution Method detects the minimal inhibitory concentration （MIC） of the water extract of Pomegranate Rind on H. pylori metronidazole resistant strains and susceptible strains, MIC50, MIC75, MIC90 were also calculated. Results MIC of Pomegranate Rind on H. pylori was in a range of 7. 8125 to 500 mg/mL; MIC50 of H. pylori metronidazole resistant strains and susceptible strains on Pomegranate Rind was 29. 9 mg/mL and 28.0 mg/mL respectively, MIC75 was 65. 1 mg/mL and 59. 1 mg/mL respectively, MIC90 was 131.1 mg/mL and 115.9 mg/mL respectively. The susceptibility of H. priori metronidazole resistant strains and susceptible strains on Pomegranate Rind has no statistical difference （relative median potential = 1. 072, 95% credible range from 0. 763 to 1. 513 ） . Conclusion H. pylori metronidazole resistant strains and susceptible strains was susceptible to Pomegranate Rind.