MTT法和LDH法检测T-AK细胞活性的研究

Study on Determination of Cytotoxicity of T-AK Cells with MTT Colorimetry and LDH Release Assays

采用MTT比色分析法和LDH释放法检测了CD_3单克隆抗体和白细胞介素—2共同激活的T—AK细胞的杀伤活性,并对检测方法进行了改良。结果表明:T—AK细胞对K_(562)细胞有很高的杀伤活性;MTT法和LDH法经本研究改良后应用于细胞毒活性的检测,具有简便实用、方法稳定和结果可靠的特点;经对比观察,MTT法和LDH法的检测结果具有良好的相关性,但MTT法较LDH法敏感。

The cytotoxicity of anti-CD3 monoclonal antibody and interleukin-2 costimulated killer cells-T-AK (T-activated killer)cells was detected with MTT colorimetric assay and Lactic dehydrogenase(LDH)release assay,and the determinating assays were improved in the study. The results showed that T-AK cells had potent cytolytic activity on human leukemia K562 cells. After our improvement, MTT assay and LDH assay became simple,convenient,stable and trustworthy in the determination of cytotoxicity. MTT colorimetric assay was much more sensitive than LDH release assay in determinating the cytolytic activity of tumor killer cells.