基因枪转化法获得草地早熟禾(Poa pratensis L.)转基因植株

Transformation of Kentucky Bluegrasss (Poa pratensis L.) by Particle Bombardment

将与植物抗旱耐盐有关的BADH-CMO双基因、CMO单基因、DREB1A单基因三种外源基因利用基因枪法分别轰击草地早熟禾的胚性愈伤组织,在附加100mg/L潮霉素的继代培养基筛选和附加50mg/L潮霉素的再生培养基中壮苗1个月,将抗性植株移栽到花盆中。经过PCR检测、Southern杂交分析,证明BADH-CMO双基因、CMO基因、DREB1A基因已经成功整合到草地早熟禾的植物基因组中。

BADH-CMO double gene, CMO gene and DREB1A gene were transformed respectively to embryonic callus of Kentucky bluegrass by particle bombardment. Then the embryonic callas of kentucky bluegrass were put in meclium for subculture which is mixed with 100mg/L hygromycin and the meclium for plantlet regeneration which mixell with 50mg/L hy gromycin about one month. Thus, the hygromycin-selectecl plants were obtained and were transplantecl into flowerpots. The results of the PCR and Southern blot analysis indicated that the DREB1A gene, CMO gene and BADH-CMO double-gene were integrated into the genomic DNA of Kentucky bluegrass.