实时PCR分析△^5脱饱和酶在花生四烯酸合成中的作用

Analyzing Role of △^5 Desaturase in Biosynthesis of Arachidonic Acid Using Real Time PCR

花生四烯酸是人体的必需脂肪酸，具有独特的生物活性。△^5脱饱和酶是花生四烯酸生物合成途径中催化二高-γ-亚麻酸脱饱和为花生四烯酸的酶。通过实时定量PCR技术，检测了△^5脱饱和酶在不同花生四烯酸产量的高山被孢霉（Mortierella alpina）菌株M10、M6和M23中的mRNA表达水平，以及在高产花生四烯酸菌株M6培养过程中的mRNA表达水平的动态变化。发现岔脱饱和酶基因的mRNA表达水平与花生四烯酸的产生之间存在明显的线性关系，表明△^5脱饱和酶在高山被孢霉花生四烯酸合成途径中起到了非常重要的作用。

Arachidonic acid is an essential fatty acid in human nutrition and a biogenetic precursor of the biologically active prostaglandins and leukotrienes. △^5desaturase catalyzes the △^5dehydrogenation of di-homo-γ- linolenic acid to form arachidonic acid in biosynthetic pathway of arachidonic acid. Using real time PER technology, the transcriptional expression levels of gene encoding △^5 desaturase in three MortiereUa alpina strains M10, M6 and M23, and in different growth phase of high arachidonic acid yielding strain M6, were determined. Results showed that there was a distinct corelafionship between mRNA transcript level of △^5 desaturase gene and biosynthesis of arachidonic acid. Results indicated that △^5 desaturase plays an important role in arachidonic acid biosynthesis.