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特异性鼠抗人交联纤维蛋白ScFv基因的克隆、表达及ScFv-Scu-PA-32K融合蛋白的构建 被引量:1

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摘要 特异性鼠抗人交联纤维蛋白ScFv基因的克隆、表达及ScFv┐Scu┐PA┐32K融合蛋白的构建黄君健黄翠芬俞炜源军事医学科学院生物工程研究所北京100850为了获得具有导向作用的特异性抗体,采用目前最新的噬菌体抗体库技术,制备特异性抗人交联纤维蛋白单...
出处 《军事医学科学院院刊》 CSCD 北大核心 1996年第4期277-277,共1页 Bulletin of the Academy of Military Medical Sciences
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  • 1胡川闽,刘彦仿,陈志南,隋延仿,仇凯,徐力青.两株新的抗肝细胞肝癌单克隆抗体的制备及特异性鉴定[J].单克隆抗体通讯,1994,10(2):16-20. 被引量:7
  • 2[美]萨姆布鲁克(Sambrook,J·)等 著,金冬雁等.分子克隆实验指南[M]科学出版社,1992.
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  • 4Isaacs JD.The antiglobulin response to therapeutic antibodies.Seminars in Immunology 1990; 2: 449 2 Jones PT, Dear PH. Footre J et al. Replacing the complementarity-determining regions in a human antibody with those froma mouse. Nature . 1986
  • 5Kettleborough CA,Saldanha J,Heath VJ et al.Humanization of a mouse monoclonal antibody by CDR-grafting: The importance of framework residues on loop conforma-tion. Protein Engineering . 1991
  • 6Molloy PE,Graham BM,Cupit PM et al.Expression and purification strategies for the production of singlechain antibody and T-cell receptor fragments in E.coli. Molecular Biotechnology . 1995
  • 7Buchner J,Pastan I and Brinkmann U.A metbod for increasing the yields of properly folded recombinant fusion proteins: single-chain immunotoxins from renaturation of bacterial inclusion bodies. Ana Biochem . 1992
  • 8Pluckthun A.Antibody engineering: advances from the use of E.coli expression system. Bio Technology . 1991
  • 9Duenas M,Vazquez J,Ayala M et al.Intra-and extra cellular expression of an seFv antibody fragments in E.coli: Effect of bacterial strains and pathway engineering using GroES/L chaperonins. Biotechniques . 1994
  • 10Knappik A and Pluckthun A.Engineered turns of a recombinant antibody improve its in vivo folding. Protein Engineering . 1995

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