摘要
Objective： To investigate the pathological changes following the focal cerebral ischemia and reperfusion in rats and the effect of acupuncture on them. Methods： A model of ischemia and reperfusion was established by thread occlusion, and the brain activating acupuncture was applied, and then the morphological changes of the cerebral cortex on the ischemic side were observed under electron and light microscopes. Results： Cerebral ischemia and reperfusion could lead to the structural injury of neurons, gliacytes and capillary vessels in rat＇s brain, and acupuncture could improve the ultra-structural injury in the area around the cerebral ischemia. It was also found that acupuncture intervention at 3 hours following reperfusion was more ideal than that at any other time points. Conclusion： Acupuncture has a protective effect on the ultra-structural injury of neurons associated with focal cerebral ischemia and reperfusion in rats, and acupuncture given within 3 hours following reperfusion can produce a satisfactory effect.
目的:探讨局灶性脑缺血再灌注大鼠病理学改变以及针刺的干预作用。方法:采用线栓法建立缺血再灌注模型,应用"醒脑开窍"针法并通过电镜与光镜来观察缺血侧大脑皮层形态结构的变化。结果:脑缺血再灌注可引发大鼠脑神经元、胶质细胞、毛细血管等结构损伤,针刺可改善脑缺血周围区超微结构损伤。并且发现在3 h 时间点给予针刺干预较其他时间点理想。结论:针刺对局灶性脑缺血再灌注大鼠脑神经细胞超微结构损伤具有保护作用。在3 h 内给予针刺干预可以收到满意的效果。
