大肠腺瘤-腺癌序列过程中PTEN表达的变化及意义

The Expression of PTEN in Colorectal Adenoma-Carcenoma Sequence

目的:研究大肠腺瘤-腺癌序列过程中PTEN蛋白表达的变化,以阐明它在大肠腺癌发生发展中的作用。方法:采用二步法免疫组织化学染色法检测了119例手术切除及内镜活检标本(腺瘤38例,腺癌67例)和14例正常肠粘膜内PTEN蛋白的表达。结果:①大肠腺瘤-腺癌序列过程中PTEN蛋白表达从正常肠粘膜→腺瘤→腺癌逐渐降低;②在大肠患者中有淋巴结转移和无淋巴结转移者PTEN的表达无显著差异(P>0.05)。结论:PTEN的过度表达是大肠腺癌发生发展过程中的早期事件,并可能在大肠腺瘤-腺癌序列的发展过程中起重要作用。

Objective：To investigate the expression of PTEN in colorectal adenoma-carcenoma sequence,and the role of PTEN in colorectal adenoma-carcenoma sequence. Methods： One hundred nineteen intestinal biopsy specimens（38 of adenoma, 67 of colorectal adenocarcinoma） and 14 normal subjects were taken. The expression of PTEN protein in intestinal mucosa cells were detected by immunohistochemical staining. Results：①From normal mucosa,adenoma to colorectal adenocarcinoma, the expression of PTEN protien increased gradually;② In colorectal adenocarcinoma, there was not significantly difference between patients with metastasis and nonmetastasis for expression of PTEN（P〉0.05）. Conclusion： The over expression of PTEN was an earlier event of colorectal adenoma-carcenoma sequence and may be an important integral component of the genesis of colorectal adenoma and carcinomas.