HPLC法测定奈韦拉平的血药浓度

Determination of Nevirapine in Human Plasma by High Performance Liquid Chromatography

目的：建立高效液相色谱法测定奈韦拉平血药浓度的方法。方法：采用Hypersil C18柱（150min×4．6mm,0．5μm）；流动相：乙腈：0．01mol／L磷酸二氢钠（含0．01mol／L三乙胺，磷酸调pH至5.O）。18。82；流量：1．0mL／min；检测波长：240nm。结果：线性范围0．1～5．0mg／L（r=0．9996）；最低检测浓度为0．1mg／L；日内、日间相对标准差（RSD％）均小于10％；低、中、高三个浓度的提取回收率分别为99．98％、99．41％和100.53％。结论：该法简便、快速、准确，专属性高，适用于奈韦拉平药动学和生物等效性研究。

Objective：To establish a method for the determination of nevirapine in human plasma using HPLC. Methods： The analytic column was Hypersil C18 column（150 mm×4. 6 mm,0. 5μm）. The mobile phase was acetonitrile-0.01 mol/L NaH2PO4 （including 0.01mol/L acetamide and adjust pH to 5.0 with H3PO4）. The flow rate was 1.0 mL/min. The detection wavelength was 240 nm. Results：Nevirapine concentration presented a good linear range of 0.1-5.0 mg/L（r =0. 9996）had the limit of quantitation was 0.1 mg/L. The relative standard deviations of withinday and between-days were less than 10%. The recovery was between 99.41% and 100.53%. Conclusion： The method is rapid, simple and accurate for the determination of nevirapine in human plasma. It is suitable for the pharmacokinetics and bioavailability study of nevirapine.