二色胡枝子组织培养的研究

Studies on tissue culture of Lespedeza bicolor

为探索建立植株再生系统技术,以二色胡枝子种子为材料,对该树种组织培养进行了研究。结果表明,以0.1%升汞对种子灭菌2 min,发芽率较高,污染率仅为5%;基础培养基为1/2 MS,直立苗比例达到80%以上,显著高于MS和B5培养基;在增殖培养中,BA对分化系数影响最大,NAA其次,ZT的作用不明显,适宜的增殖培养基为1/2 MS+BA 1.0 mg/L+NAA 0.01 mg/L,分化系数可达3.15;随着NAA浓度提高,对生根抑制作用加强,其浓度不宜超过0.5 mg/L,IBA对生根有促进作用,浓度以IBA 1.5 mg/L为宜。

Tissue culture of Lespedeza bicolor was studied in this experiment to develop a regeneration system. The results showed that the best disinfection time for Lespedeza bicolor seeds was 2 min with 0.1% HgCl2, in which shooting percent reached 32.8 %, while polluted percent of the seeds was just 5 %. According to the results of experiment with orthogonal design, the concentration of 6 - BA was the main factor which had a significant effect on the index of differentiation of buds. NAA was also effective as the second important factor and ZT seemed to have no effect. The optimum differentiation culture medium was 1/2 MS ＋ BA 1.0 mg/L ＋ NAA 0.01 mg/L, of which the index of generation could reach 3.15. The concentrations of NAA should be lower than 0.5 mg/L because inhibiting effect on rooting of plants became stronger along with its increasing. IBA had promoting effect on rooting of plants, and the optimum concentration was 1.5 mg/L.