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cDNA末端扩增技术的研究进展 被引量：8

Research progress of rapid amplification of cDNA ends
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摘要 cDNA末端扩增技术是一种以PCR反应为基础,快速获得已知cDNA序列3′端和5′端的方法,是克隆全长cDNA序列的主要手段之一。本文从RACE原理出发,对RACE技术3个关键步骤(反转录、TdT加尾、PCR扩增)的优化和改良措施进行了透彻分析,并详细阐述RACE的最新研究进展———“电子”cDNA克隆。 Rapid amplification of cDNA ends is a method obtaining 3＇ ends and 5＇ ends of known cDNA fragments based on PCR reaction, and one of the primary means cloning cDNA fragments. Beginning with the principle this article analyses the three critical steps （transcription, TdT tailing, PCR amplification） of RACE technique in detail, and expatiates the latest RACE techniques （Marathon - PCR, SMART - RACE, In silico cloning）.
作者 郝敏 谷守芹 韩建民 董金皋
机构地区 河北农业大学真菌毒素实验室 保定师范专科学校生物系
出处 《河北林果研究》 2006年第2期157-161,共5页 Hebei Journal of Forestry and Orchard Research
关键词 RACE 全长CDNA序列 PCR 反转录 RACE full - length cDNA PCR transcription
分类号 S791.117.05 [农业科学—林木遗传育种]
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参考文献25

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二级参考文献87

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共引文献72

同被引文献114

引证文献8

二级引证文献46

河北林果研究
2006年 第2期

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