摘要
目的 测定刺五加浸膏中异秦皮啶的含量。方法 采用高效液相色谱法,C18色谱柱为固定相,乙腈-水(60:40)为流动相,检测波长为345nm。结果 异秦皮啶的含量在0.44-2.2μg范围内,峰面积与其浓度呈良好线性关系(r=0.9997).平均回收率为102.95%,RSD为2.4%(n=6)。结论 该法简单准确,重现性好。
Objective To determine the content of isofrasidin in Ciwujia Jingao. Methods HPLC was used. The stationary, phase was Spherixorb C18column. The moble phase was acetonitrile-water(60: 40). The UV detection wavelength was 345 nm. Results There was a good linear relationship within the concentration rang of 0.44 - 2.2μg of isofraxidin Ciwujia Jingao( r = 0. 999 7 ). The average recovery was 102.95% ( n = 6) ,RSD = 2.4%. Conclusion The method is simple,accurate and easy to operate.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第9期I0001-I0001,共1页
Lishizhen Medicine and Materia Medica Research